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Image Search Results
Journal: Heliyon
Article Title: Persistent viruses in mosquito cultured cell line suppress multiplication of flaviviruses
doi: 10.1016/j.heliyon.2018.e00736
Figure Lengend Snippet: Identification of viruses infected in mosquito cultured cell line C6/36. A : Schematics of Shinobi tetravirus (SHTV) and Menghai rhabdovirus (MERV) genomic sequences. Encoded putative genes and their expected sizes are presented (RdRp: RNA-dependent RNA polymerase, VPg: Viral protein genome-linked, CP: capsid protein, N: nucleoprotein, P: phosphoprotein, M: matrix protein, G: glycoprotein, and L: RNA-dependent RNA polymerase). B : Phylogenetic analysis of SHTV and other viruses containing Permutotetraviridae . The amino acid sequences of putative RdRp were aligned and then analyzed using a maximum-likelihood method. Bootstrap replications are shown on the branches. C : Phylogenetic analysis of MERV and other rhabdoviruses. The amino acid sequences of L proteins were analyzed as described. D : Detection of SHTV or MERV in C6/36 JCRB strain and ECACC strain. The viral genome was determined by RT-PCR with the template RNA extracted from cell culture supernatant or cultured cells. PCR amplification from genomic DNA extracted from cultured cells were also determined. J: C6/36 JCRB strain, E: C6/36 ECACC strain. The original image is shown in Supplementary Material 1.
Article Snippet: The growth kinetics of flaviviruses in
Techniques: Infection, Cell Culture, Genomic Sequencing, Reverse Transcription Polymerase Chain Reaction, Amplification
Journal: Heliyon
Article Title: Persistent viruses in mosquito cultured cell line suppress multiplication of flaviviruses
doi: 10.1016/j.heliyon.2018.e00736
Figure Lengend Snippet: C6/36 cells of JCRB and ECACC strains showed different susceptibility to flavivirus infection. Growth kinetics of arboviruses in C6/36 cell lines. A : C6/36 cells of either JCRB or ECACC strain were infected with Zika virus (ZIKV), Japanese encephalitis virus (JEV), or Dengue virus (DENV) at a multiplicity of infection (MOI) of 0.01, and the titers in the supernatants were determined by plaque assay. B : C6/36 cells of either JCRB or ECACC strain were infected with Getah virus (GETV) or Sindbis virus (SINV) with MOI = 0.001. Red lines show the titer in C6/36 JCRB strain and black lines show that in C6/36 ECACC strain. The experiment was triplicated, and the standard deviations are also indicated. Asterisks showed the results of t -test for the data group at each time points (* p < 0.05, ** p < 0.01).
Article Snippet: The growth kinetics of flaviviruses in
Techniques: Infection, Virus, Plaque Assay